ACTIVITY AND CHARACTERISTICS OF MALTASE FROM COTYLEDONS OF MARROWS (Cucurbita pepo L.)
نویسندگان
چکیده
منابع مشابه
l-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.
ORNITHINE: 2-oxoacid aminotransferase (EC 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (Cucurbita pepo, L.) seedlings. The pH optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the Michaelis constants are 4.7 mm and 6.3 mm, respectively. The enzyme has a molecular weight of 48,000 ...
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High molecular weight genomic DNA was isolated from excised marrow cotyledons (Cucurbita pepo L., zucchini) using high concentration of ethidium bromide (400 μg.ml–1) in the grinding buffer and phenol deproteinization of the crude nuclear pellet. This preparation of DNA was free of proteins but contained polysaccharide contaminations which did not allow complete digestion with a number of restr...
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René Renato Balandrán-Quintana1, Ana María Mendoza-Wilson2, Irasema Vargas-Arispuro2 and Miguel Angel Martínez-Téllez2* Universidad Autónoma de Chihuahua, Facultad de Ciencias Químicas, Ciudad Universitaria s/n, A.P. 1542-C Chihuahua, Chihuahua, México Centro de Investigación en Alimentación y Desarrollo, A.C., Coordinación de Tecnología de Alimentos de Origen Vegetal, Km 0.6, Carretera a La Vi...
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Cucumber mosaic virus infection of its susceptible host Cucurbita pepo results in a program of biochemical changes after virus infection. Applying a spatial analysis to expanding infected lesions, we investigated the relationship between the changes in enzyme activity and gene expression. Patterns of altered expression were seen that could not be detected by RNA gel blot analysis. For all the h...
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Lateral roots of intact summer squash seedlings (Cucurbita pepo L.) were used to quantify the effects of boron deficiency on DNA synthesis, protein synthesis, and respiration. The temporal relationship between changes in these metabolic activities and the cessation of root elongation caused by boron deprivation was determined. Transferring 5-day-old squash seedlings to a hydroponic culture medi...
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ژورنال
عنوان ژورنال: Journal of Plant Production
سال: 2011
ISSN: 2090-374X
DOI: 10.21608/jpp.2011.85769